Scientist/Senior Scientist, Immunology

@ NewLimit

About NewLimit

NewLimit is a biotechnology company working to radically extend human healthspan. We’re developing medicines to treat age-related diseases by reprogramming the epigenome, a novel therapeutic mechanism to restore regenerative potential in aged and diseased cells. We leverage functional genomics, pooled perturbation screening, and machine learning models to unravel the biology of epigenetic aging and disease using experiments of unprecedented scale.

Position

NewLimit is seeking an immunologist with experience in functional genomics to join our Immunology team. One of our initial therapeutic programs is developing an epigenetic reprogramming intervention to rejuvenate aged T lymphocytes to improve responses to infectious disease. In this role, you will develop and perform immunology assays, contribute to team decision making, and serve as a subject matter expert to enable our therapeutic development efforts.

Responsibilities

• Collaborate with Single Cell Technology, Epigenetic Editing, and Predictive Modeling teams to design, build, and test epigenetic reprogramming interventions for aged and exhausted lymphocytes.
• Develop scalable functional and phenotypic assays to predict therapeutic activity targeting T cells.
• Evaluate epigenetic reprogramming payloads using ex vivo and in vivo functional assays to select therapeutic candidates.
• Develop methods for in vitro and in vivo delivery of nucleic acid payloads to primary T cells in collaboration with the Epigenetic Editing team.
• Analyze and interpret experimental data to drive pre-clinical decision making.

Requirements

• Ph.D. in cell biology, immunology or a related field or equivalent industry experience (5+ years).
• Expertise in multi-dimensional flow cytometry.
• Experience with primary immune cell isolation, ex vivo culture, and functional assays.
• Experience with molecular cloning and ex vivo transgenic manipulation of immune cells (e.g. viral transduction, non-viral genome editing and/or transgene delivery).

Nice to Have

• Experience studying T cell responses in vivo using murine infection or tumor models.
• Experience with pooled screening in vitro or in vivo using bulk or single cell read-outs (e.g. CRISPR fitness screens, Perturb/CROP-seq).
• Familiarity with functional genomics, next generation sequencing assays, and/or single cell genomics methods.
• Familiarity with basic computational tools for data analysis (e.g. Python, R).

Benefits

• Health, dental, and vision insurance.
• 401(k) with company match.
• Industry-leading paid time off (20 days/year for all employees + holidays).
• Paid parental leave.
• Meals and snacks on-site.

Salary Range: $113,000 to $171,000. Compensation depends on multiple factors including qualifications and experience. NewLimit offers a competitive benefits package. Learn more at: https://www.newlimit.com/careers.

Key skills/competency

• Immunology
• Functional Genomics
• Epigenetics
• Flow Cytometry
• Assay Development
• Cell Biology
• Data Analysis
• Molecular Cloning
• In Vivo Studies
• Collaboration