Ingénieur en techniques biologiques - H/F
INSERM · Nice, Provence-Alpes-Côte d'Azur, France
- On site
- Full-time
- €45,000 / year
- Nice, Provence-Alpes-Côte d'Azur, France
Job highlights
- Research engineer in biological techniques.
- Conduct cell and molecular biology experiments.
- Analyze multi-omics data using bioinformatics.
- Work with human and murine cell models.
- Contribute to scientific publications and reports.
About the role
About Inserm
Inserm is the only French public research organization entirely dedicated to biological research, medical research, and population health research. It has research laboratories across the country, organized into 12 Regional Delegations. Our institute brings together 15,000 researchers, engineers, technicians, and administrative staff, with a common goal: to improve everyone's health through the advancement of knowledge about life and diseases, innovation in treatments, and public health research.
Joining Inserm means becoming part of an institute committed to parity and professional equality, diversity, and support for employees with disabilities, from recruitment through the entire career. To ensure well-being at work, Inserm actively pursues a policy regarding working conditions, focusing particularly on a healthy balance between personal and professional life.
In 2016, Inserm received the European HR Excellence in Research label and committed to evolving its practices for recruiting and evaluating researchers.
The Position
The engineer will carry out their activities within the C3M (Inserm U1065) in Nice.
Missions
- Participate in the design, implementation, and analysis of research projects in cell and molecular biology.
- Conduct experiments on human and murine cellular models.
- Ensure the analysis and integration of multi-omics data (RNAseq, ATACseq, single-cell RNAseq, proteomics).
- Develop and optimize bioinformatics analysis pipelines.
- Contribute to the scientific valorization of results (reports, publications, presentations).
Main Activities
- Culture and maintenance of cell lines, primary cells from donors and patients.
- Preparation of biological samples.
- Purification of organelles and preparation of cellular extracts.
- Performance of molecular and cellular biology techniques: Western blot, immunofluorescence, RNA/DNA/protein extraction.
- Preparation and quality control of samples for transcriptomic and proteomic analyses.
- Bioinformatic analysis of RNAseq, ATACseq, single-cell RNAseq, and proteomic data.
- Statistical processing and visualization of biological data.
- Participation in database management and archiving of experimental results.
Associated Activities
- Scientific and technological watch in cell biology and bioinformatics.
- Participation in team meetings and interdisciplinary collaborations.
- Writing experimental protocols and technical reports.
Profile Sought
Knowledge
- Cellular and molecular biology.
- Cell culture of cell lines and primary cells.
- Molecular and protein biology techniques (Western blot, immunofluorescence, RNA/DNA/protein extraction).
- Transcriptomics and epigenomics: RNAseq, ATACseq, single-cell RNAseq.
- Analysis of proteomic data.
- Bioinformatics and statistics applied to omics data.
- Experience in bioinformatics analysis of transcriptomic and single-cell data using dedicated tools (Seurat, Scanpy, RNAseq/ATACseq pipelines).
Skills
- Design and conduct experiments in cell and molecular biology.
- Handle human primary cells and murine models in compliance with regulatory procedures.
- Perform multi-omics analyses and interpret biological results.
- Develop and use bioinformatics analysis pipelines.
- Use statistical tools and data visualization software.
- Write scientific reports and present results concisely.
- Work in a team within a multidisciplinary environment.
Aptitudes
- Scientific rigor and organizational skills.
- Analytical and synthesis skills.
- Autonomy and initiative.
- Ability to work in a team and collaborate.
- Adaptability and scientific curiosity.
- Good written and oral communication skills.
Specifics/Constraints of the Position
- Position involves handling human cells and murine models.
- Compliance with ethics, biosafety rules, and regulatory procedures.
Desired Experience
- Experience in cell and molecular biology applied to biomedical research.
- Experience in bioinformatics analysis of omics data.
- Experience desired in RNAseq, ATACseq, single-cell RNAseq, and/or proteomics.
- Experience with human primary cells and murine models would be appreciated.
Key skills/competency
- Cell Biology
- Molecular Biology
- Bioinformatics
- Multi-omics Data Analysis
- RNAseq
- ATACseq
- Single-cell RNAseq
- Proteomics
- Statistical Analysis
- Scientific Communication
Skills & topics
- Biological Techniques Engineer
- Cell Biology
- Molecular Biology
- Bioinformatics
- Multi-omics
- RNAseq
- ATACseq
- Single-cell RNAseq
- Proteomics
- Statistical Analysis
- Research Engineer
- Biomedical Research
- Nice
- Inserm
- France
How to get hired
- Tailor your resume: Highlight relevant experience in cell biology, molecular biology, and bioinformatics, using keywords from the job description.
- Showcase your skills: Emphasize your proficiency in techniques like Western blot, RNAseq, and ATACseq, and your experience with omics data analysis.
- Demonstrate your research aptitude: Prepare to discuss your scientific rigor, analytical skills, and ability to design and conduct experiments.
- Prepare for technical questions: Be ready to explain your experience with bioinformatics pipelines, statistical analysis, and data visualization tools.
- Understand Inserm's mission: Show your commitment to improving health through scientific advancement and research.
Technical preparation
Master cell culture and molecular biology techniques.,Practice analyzing RNAseq, ATACseq, and proteomics data.,Develop proficiency in bioinformatics pipeline development.,Strengthen statistical analysis and data visualization skills.
Behavioral questions
Describe a complex research project you managed.,How do you handle unexpected experimental results?,Provide an example of effective scientific communication.,How do you stay updated with scientific advancements?
Frequently asked questions
- What is the primary focus of the Biological Techniques Engineer role at Inserm?
- The primary focus of the Biological Techniques Engineer role at Inserm is to participate in research projects in cell and molecular biology, conduct experiments on human and murine cell models, analyze multi-omics data, and develop bioinformatics pipelines.
- What are the key scientific techniques and data types involved in this position at Inserm?
- This position involves techniques such as Western blot, immunofluorescence, RNA/DNA/protein extraction, and cell culture. You will also work with multi-omics data including RNAseq, ATACseq, single-cell RNAseq, and proteomics.
- What specific bioinformatics tools or pipelines are important for this Biological Techniques Engineer role at Inserm?
- Experience with bioinformatics analysis of transcriptomic and single-cell data using tools like Seurat, Scanpy, and dedicated RNAseq/ATACseq pipelines is important for this role at Inserm.
- Does Inserm's Biological Techniques Engineer position involve working with human or animal subjects?
- Yes, this position involves working with human primary cells from donors and patients, as well as murine models, in compliance with all ethical, biosafety, and regulatory procedures.
- What kind of scientific output is expected from the Biological Techniques Engineer at Inserm?
- The engineer will contribute to the scientific valorization of results, which includes writing reports, preparing publications, and presenting findings at scientific meetings.
- What is Inserm's commitment to employee well-being and professional development, relevant to this role?
- Inserm is committed to professional equality, diversity, and supporting employees with disabilities. They also focus on work-life balance and have been recognized with the HR Excellence in Research label.
- What is the location for the Biological Techniques Engineer position at Inserm?
- This position is located at the C3M (Inserm U1065) in Nice, France.